Detection of the Effect of Microwave Radiation on the Phenotypic and Molecular Characteristics of Radish Plant

Abstrak

The current study aimed to investigate the effect of microwave radiation on the molecular properties of radish. And detection of mutations resulting from exposure to microwave radiation on the genetic material of radish plant using RAPD-PCR technique. Samples were collected from the plants one month after the date of planting from all treatments, where (4-5) young leaves were taken from the growing apex and placed in special marked bags and transported directly to the laboratory to conduct the process of DNA isolation from them. DNA was extracted from the plant using the column tube method. The results of The RAPD indicators are characterized by their sensitivity to any change in the components of their interactions. Primer P1 molecular sizes ranged between 100bp-1500bp, and the number of produced bundles reached 31 bundles, ranging between 1-7 bundles per sample. Primer P3 molecular sizes ranged between 250 pb-1250, and the number of produced bundles reached 45 bundles, ranging between 1-9 bundles per sample. Primer P4 molecular sizes ranged between 200bp-1250bp, and the number of produced bundles reached 41 bundles, ranging between 1-8 bundles per sample. Primer P5 molecular sizes ranged between 150bp-1500bp, and the number of produced bundles reached 22, ranging between 1-7 bundles per sample. Primer P8 molecular sizes ranged between 150bp-1250bp, and the number of produced bundles reached 30 bundles, ranging from 1-6 bundles per sample. Primer P12 molecular sizes ranged between 250bp-1500bp, and the number of bundles produced reached 42 bundles, ranging between 1-12 bundles per sample. Primer P16 molecular sizes ranged between 200 bp - 1400 bp, and the number of produced bundles reached 16, whose molecular sizes ranged between 1-6 for one sample.